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Brain Neurosecretory Cytokines: Immune Response and Neuronal by Armen A. Galoyan

By Armen A. Galoyan

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"Brain Neurosecretory Cytokines is a superb ebook, a veritable goldmine of data, on the leading edge of neuroscience. it may stimulate principles for destiny instructions in neuroscience for years yet to come. This quantity could be hugely beneficial for clinical libraries, neuroscientists, endocrinologists and graduate scholars in those fields." (Arthur S. Brecher, developments in Endocrinology & Metabolism, Vol. sixteen (6), 2005)

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Example text

Those facts let us suppose that PRP-l can enhance the expression of human growth hormone (GH) from mouse fibroblasts transfected with human GH gene (clone BALB/c-GH). It is known that GH is important for the development and functioning of the immune system'". It was demonstrated that at low GH level (dwarfness and other pathologies) the inhibition of humoral and cellular chains of the immune response takes place l13 • In this respect the elucidation of the possibility of GH stimulation by hypothalamic immune modulators was of interest.

B) Dark brown PRP-l-positive granules in the germinal center of follicle. Magnification x 1000. In the germinal centers of follicle between developing B cells and accessory cells, numerous dark-stained spherical granules are visible in a high power microscope in the intracellular substance and cell surfaces (Fig. 20b). DISCOVERY OF NEW NEUROSECRETORY CYTOKINES 31 In the sections with toluidine blue staining, the majority of the granules show metachromatic reaction, which are perhaps the granules of degranulated mast cells 109.

Jll • ill · :,\GH (100 ~ ~1I ) •Vasopressin fl O' MI I . 'GH +Vasopressi» PRP -Oxytocin (10" M) Figure 21. Effect ofNGH and neuropeptides on TNF-a activity of the NOH lysate. 25 1 10 · I E ]! : 's U .. ~ '0 Ii.. z o. ' Diluuon of GN, ~ -, OO~I IIIlI -50 ~1 • ·25~1 ~ . , Dill Figure 22. Effect ofNGN lysate on the expression ofTNF-a by LPS-stimulated macrophages . : ;; s. ~ IIIs Control with out ~ . 7 :'f) • 7 ~ -PRP. 7 ~I) Figure 23. Effect ofNGN and polypeptides on the secretion ofTNF-a by LPS-stimulated astrocytes.

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