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9th International Conference on Practical Applications of by Ross Overbeek, Miguel P. Rocha, Florentino Fdez-Riverola,

By Ross Overbeek, Miguel P. Rocha, Florentino Fdez-Riverola, Juan F. De Paz

This complaints offers fresh functional functions of Computational Biology and Bioinformatics. It comprises the complaints of the ninth foreign convention on
Practical purposes of Computational Biology & Bioinformatics held at college of Salamanca, Spain, at June 3rd-5th, 2015. The overseas convention on functional functions of Computational Biology & Bioinformatics (PACBB) is an annual foreign assembly devoted to rising and not easy utilized examine in Bioinformatics and Computational Biology. organic and biomedical examine are more and more pushed by means of experimental suggestions that problem our skill to examine, approach and extract significant wisdom from the underlying information. The remarkable features of subsequent iteration sequencing applied sciences, including novel and ever evolving exact different types of omics info applied sciences, have positioned an more and more complicated set of demanding situations for the growing to be fields of Bioinformatics and Computational Biology. The research of the datasets produced and their integration demand new algorithms and methods from fields equivalent to Databases, records, facts Mining, computing device studying, Optimization, desktop technology and synthetic Intelligence. truly, Biology is a growing number of a technological know-how of data requiring instruments from the computational sciences.

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D. Pai Neto ⋅ R. Moresco ⋅ L. S. Zeggio ⋅ M. com L. Specht Environmental Military Police, Florianopolis, Brazil C. Costa ⋅ M. Rocha Centre Biological Engineering School of Engineering, University of Minho, Braga, Portugal © Springer International Publishing Switzerland 2015 R. Overbeek et al. M. Tomazzoli et al. Importantly, the discrimination based on PCA could be improved by using the dataset of the fingerprint region of phenolic compounds (λ = 280−350 ηm), suggesting that besides the biological activities presented by those secondary metabolites, they are also relevant for the discrimination and classification of that complex matrix through bioinformatics tools.

Propolis samples (500 mg) were added of 25 mL 70 % ethanol (v/v) and incubated (24 h, darkness). The extracts were filtered on cellulose support under vacuum, completing the final volume to 25 mL with 70 % EtOH. The absorbances of the hydroalcoholic extracts were measured on a UV-visible spectrophotometer (Gold Spectrum lab 53 UV-Vis spectrophotometer, BEL photonics, Brazil) using a spectral window of 280 to 800 ηm (2 ηm resolution/ data point). 3 Statistical and Chemometric Analysis The UV-Vis data set of the propolis hydroalcoholic extract was processed considering the definition of the spectral window of interest (280–800 ηm), baseline correction, normalization, and optimization of the signal/noise ratio (smoothing).

The metabolic fingerprinting, one of the metabolomics branches, is a high-throughput, rapid, global analysis to discriminate between samples of different biological status or origin, where metabolites quantification and identification are not employed [12, 13]. , similar or discrepant traits. UV-Vis scanning spectrophotometry requires little sample amount and preparation and rapidly provides valuable and robust information about the presence of particular classes of metabolites, such as flavonoids, carotenoids, chlorophylls and chalcones [14].

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